Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collections: Workshop Summary (2006)
Chapter: Appendix C: Agenda
Appendix C
Agenda
Keck Center
National Academies
500 Fifth Street, N.W.
Washington, D.C.
May 8, 2006
|
8:30-9:00 a.m. |
Opening Remarks and Introduction Ann Bucklin and Donald Crothers, Cochairs |
|
8:30-8:45 |
Purpose of the Workshop Scott Miller |
Session 1: State of DNA in Biological Samples after Exposure to Formalin
-
What is the state of preservation of DNA in the presence of formalin? Are the DNA chains intact or broken? Does formalin denature DNA or is it the process of extraction that is fragmenting the DNA? Are the nucleotides at each site being preserved or altered?
-
How can the physical and chemical states of the DNA-formalin cross-linkages be better characterized? What additional information on these cross-linkages is needed?
Moderator: Donald Crothers
Rapporteur: Timothy O’Leary
|
9:00-10:00 |
Participant Brieflngs Participants share their knowledge of and experience with assessing the condition of DNA in biological samples flxed in formalin |
|
10:00-10:30 |
Open Discussion |
|
10:30-10:45 |
Break |
|
10:45-11:30 |
Open Discussion |
Session 2: Optimization of DNA Sequence Information from the Samples
-
What new chemical and physical methods for DNA extraction should be tested, beyond those that have already been applied to formalin-flxed tissue?
-
In what ways and to what extent can fragmented DNA be repaired physically and chemically after extraction from formalin?
Moderator: Ann Bucklin
Rapporteur: Charles Cantor
|
11:30-12:30 p.m. |
Participant Brieflngs Participants share their knowledge of and experience with optimization of DNA sequence information from formalin-flxed samples |
|
12:30-1:30 |
Lunch |
|
1:30-3:45 |
Open Discussion |
|
3:45-4:00 |
Break |
Session 3: Bioinformatics for Reconstructing DNA Sequences
-
Can bioinformatics techniques be used to reconstruct the original sequence in silico from the DNA fragments recovered from formalin?
Moderator: Christoffer Schander
Rapporteur: Neil Hall
|
4:00-4:20 |
Participant Brieflngs Participants share their knowledge of and experience with bioinformatics for restructuring DNA sequences |
|
4:20-5:20 |
Open Discussion |
|
5:20-5:30 |
First-day Summation |
May 9, 2006
Session 4: The Path towards Effective Retrieval of Genomic Information from Formalin-Fixed Samples
Moderators: Donald Crothers and Ann Bucklin
|
8:30-9:15 a.m. |
Summary of Sessions 1-3 Timothy O’Leary, Charles Cantor, and Neil Hall, Rapporteurs |
|
9:15-10:30 |
Open Discussion |
|
10:30-10:45 |
Break |
|
10:45-11:45 |
Open Discussion (cont’d) |
|
11:45-12:00 |
Workshop Summation |
