Research to advance understanding of the ecology and biology of wildlife species is more important than ever as the world confronts issues ranging from biodiversity loss to the emergence of zoonotic diseases. This workshop will focus on issues associated with the unique welfare challenges of conducting research with biologically diverse vertebrates living in varied habitats, for which guidance resources on wildlife research sometimes conflict and recommendations outlined in the current version of the Guide for the Care and Use of Laboratory Animals (8th edition) often do not apply.