There is a longstanding, bidirectional relationship between neuroscience and computer science, especially in the development of artificial intelligence (AI). As AI continues to expand, the consistent engagement of neuroscientists in conversations on the uses, regulation, implications, and public’s concern could shape the field’s trajectory. Anticipating the need for these cross-disciplinary conversations, the National Academies' Forum on Neuroscience and Nervous System Disorders hosted a 1.5-day workshop in March 2024 that convened a diverse group of experts that examined the current and potential use of AI in neuroscience and examined strategies to enhance public and regulatory understanding of AI utilization.