Microphysiological systems (MPS) are in vitro platforms composed of 3-D constructs (including spheroids, organoids, bioprinted, and tissue/organs-on-chips) of human or animal origin in micro-bioreactors that mimic the biochemical, electrical, mechanical properties of organ or tissue function. MPS are predictive tools enabling more physiologically meaningful pharmacology, toxicology and efficacy assessments in drug development. Workshop will discuss current MPS landscape, particularly in developing animal organs-on-chips and establishment of sustainable animal chip banks for large-scale use. MPS will be explored for a range of animal species/strains, including pathogen-free and gene-edited animals, and for optimizing animal model selection where whole animal models are necessary.