Supported by the Forum on Microbial Threats at the National Academies of Sciences, Engineering, and Medicine, the One Health Action Collaborative (OHAC) brings together experts from across human, veterinary, plant and environmental health to put forward strategies for unifying these disciplines in healthcare systems, disease surveillance networks, and the minds of the public at large. OHAC members brainstorm, discuss, and disseminate new ideas aimed at shifting and amplifying the conversation around One Health and its role in the health systems of the future.