A Research Strategy to Examine the Taxonomy of the Red Wolf (2020)
Chapter: Appendix D: DNA Samples from Red Wolves
Appendix D
DNA Samples from Red Wolves
This table reports published DNA samples that have been extracted from putative red wolves (Canis rufus). The committee understands that unpublished sequences are held by managers of protected populations and by research groups who are actively studying canid populations. Sources include blood and tissue samples from living animals (“Wild/Managed”) and museum specimens (CM, Carnegie Museum; UMMZ, University of Michigan Museum of Zoology; USNM, United States National Museum of Natural History).-
| Source | Age | State | County | Location | Type | Reference(s) |
|---|---|---|---|---|---|---|
| USNM 348063 | Archaeo. | AL | Madison | New Fern Cave | unknown | Unpublished |
| UMMZ 91100 | Archaeo. | AL | Jackson | B.B. Comer Bridge | mtDNA control region (450 bp) | Brzeski et al., 2016; Heppenheimer et al., 2018 |
| CM 6548 | Archaeo. | PE | Bedford | New Paris Site | mtDNA control region (450 bp) | Brzeski et al., 2016; Heppenheimer et al., 2018 |
| CM 38379 | Archaeo. | PE | Lancaster | Eschelman Site | mtDNA control region (450 bp) | Brzeski et al., 2016; Heppenheimer et al., 2018 |
| USNM 135748 | 1904 | OK | Tulsa | Red Fork | cyt b (398 bp) | Wayne and Jenks, 1991 |
| USNM 136106 | 1905 | LA | Madison | Tallulah | cyt b (398 bp) | Wayne and Jenks, 1991 |
| USNM 236542 | 1921 | AR | Yell | Stillwater | cyt b (398 bp) | Wayne and Jenks, 1991 |
| USNM 242564 | 1922 | AR | Yell | Onyx, Near | cyt b (398 bp) | Wayne and Jenks, 1991 |
| USNM 244246 | 1924 | MO | Carter | Barren | cyt b (398 bp) | Wayne and Jenks, 1991 |
| USNM 249694 | 1930 | TX | Parker | cyt b (398 bp) | Wayne and Jenks, 1991 | |
| Wild/Managed | 1970s | LA | Cameron | cyt b (398 bp), 138 restrict. sites | Wayne and Jenks, 1991 | |
| Wild/Managed | 1970s | TX | Chambers | cyt b (398 bp), 138 restrict. sites | Wayne and Jenks, 1991 | |
| Wild/Managed | 1970s | TX | Harris | cyt b (398 bp), 138 restrict. sites | Wayne and Jenks, 1991 | |
| Wild/Managed | 1970s | TX | Brazoria | cyt b (398 bp), 138 restrict. sites | Wayne and Jenks, 1991 | |
| Wild/Managed | 1970s | TX | Jefferson | cyt b (398 bp), 138 restrict. sites | Wayne and Jenks, 1991 | |
| Wild/Managed | 1970s | TX | Liberty | cyt b (398 bp), 138 restrict. sites | Wayne and Jenks, 1991 | |
| Wild/Managed | 1990s | NC | Dare | Alligator River NWR | 10 nuclear microsatellite loci | Roy et al., 1994 |
| Wild/Managed | 2000s | NC | Dare | Alligator River NWR | mtDNA cntl reg and Y chrom microsatellite data | Hailer and Leonard, 2008 |
| Wild/Managed | 2010s | NC | Dare | Alligator River NWR | 7068 nuclear SNPs | Heppenheimer et al., 2018 |
| Wild/Managed | 2010s | NC | Dare | Alligator River NWR | 48,000 nuclear SNPs | VonHoldt et al., 2011 |
REFERENCES
Brzeski, K. E., M. B. DeBiasse, D. R. Rabon, Jr., M. J. Chamberlain, and S. S. Taylor. 2016. Mitochondrial DNA variation in Southeastern pre-Columbian canids. Journal of Heredity 107(3):287–293.
Hailer, F., and J. A. Leonard, J. A. 2008. Hybridization among three native North American Canis species in a region of natural sympatry. PLoS one, 3(10), e3333.
Heppenheimer, E., K. E. Brzeski, R. Wooten, W. Waddell, L. Y. Rutledge, M. J. Chamberlain, D. R. Stahler, J. W. Hinton, and B. M. vonHoldt. 2018. Rediscovery of red wolf ghost alleles in a canid population along the American Gulf Coast. Genes 9(12):618.
vonHoldt, B. M., J. P. Pollinger, D. A. Earl, J. C. Knowles, A. R. Boyko, H. Parker, E. Geffen, M. Pilot, W. Jedrzejewski, B. Jedrzejewska, V. Sidorovich, C. Greco, E. Randi, M. Musiani, R. Kays, C. D. Bustamante, E. A. Ostrander, J. Novembre, and R. K. Wayne. 2011. A genome-wide perspective on the evolutionary history of enigmatic wolf-like canids. Genome Research 21:1294–1305.
Wayne, R. K., and S. M. Jenks. 1991. Mitochondrial DNA analysis implying extensive hybridization of the endangered red wolf Canis-rufus. Nature 351(6327):565.
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