A
Activists and protesters
beliefs and misconceptions about GM foods, 6, 7, 8, 10, 20, 71-72, 91-92, 108, 156, 187, 223, 225, 285-286, 299-300, 310, 312
big-business concerns, 151, 152-153
destruction of experiments, 146
gene flow concerns, 223, 225, 235
and Monarch butterfly, 203-209
Aegilops speltoides, 28
Aegilops squarrosa, 29, 30, 67, 68, 141
African Agricultural Technology Foundation, 296, 297
Agricultural Biotechnology Stewardship Technical Committee, 205
Agricultural development. See also Domestication of wild species;
Organic farming and foods;
Selective breeding;
Sustainable agriculture
arable farming, 30
barriers to yield increases, 303-309
and biodiversity, 240-241, 243, 315
ecological effects, 240-241, 243, 263-270, 271-272, 308
economic issues, 152-153, 310-311
fertilizer development, 49-50
Green Revolution, 3, 5, 41, 64, 264, 265-266, 300, 301, 304
hobby or firestick farming, 25-26
and human health, 43
and mutation of crops, 28, 30, 31-32, 37-38, 42, 43, 51
patents and, 133-134
plowing practices, 271-272
and population growth, 42-43, 62-63
Agriculture Canada Research Station, 236
Agrobacterium tumefasciens, 5, 83, 123-127, 129, 130, 147, 174, 225, 276, 285
Alfalfa, 276
chitinase, 183
common traits of proteins, 189
conventionally bred plants, 187-188, 190, 254
2S high-methionine albumins, 189, 190-191, 197
immunoglobulin E reaction, 185-186, 189, 191, 196
incidence in humans, 184-185, 253
lectins, 180
in microbial proteins, 184
peanuts, 185, 186, 187, 188, 189, 190
problem foods, 185, 186, 187-188, 198
reaction process, 185-186
StarLink corn case, 191-196, 197
suppression in GM foods, 198-199
testing for, 189-190, 191, 194, 196
thioredoxin and, 198
“traditional foods,” 187-188
Altieri, Miguel, 260
Aluminum tolerance, 308-309
Ambrosoli, Mauro, 133, 134, 271
American Medical Association, 161, 190
American Soybean Association, 273-274
Ames, Bruce, 253-254
Ames test for carcinogens, 253
Ammann, Klaus, 9, 17, 19, 231, 235, 269
Anderson, Edgar, 24, 29, 30, 34, 67
Animal and Plant Health Inspection Service, 146-147
Antibiotic resistance, 114, 213
marker genes, 4, 109-110, 119, 125-126, 149, 156, 160-163
Antibiotics, 111, 120, 248, 250
Aphids, 130, 138, 167, 281-283, 284, 285-286
Apple, 24, 53-54, 71, 72, 108-109, 112, 231, 259-260, 280-281
Aquinas, Thomas, 72
Arabidopsis thaliana, 5, 82, 85, 93-94, 123, 174, 298, 304
Arable farming, 30
Arber, Werner, 115
Aristotle, 68
Arkansas Children’s Hospitals, 198
Aventis, 151, 191, 195, 212, 219
Aventis CropScience, 194, 205, 297
Avery, Oswald, 79
B
Bacillus anthracis, 213-214, 254
Bacillus cereus, 193, 213, 254
Bacillus thuringiensis. See also Bt crops and pesticides
Cry proteins, 192-194, 195-196, 203, 205, 206, 207-208, 213, 225
toxins in pesticides from, 192, 202, 254
Bacteria. See also Plasmids;
individual species
allergenic proteins, 184
antibiotic resistance, 4, 109-110, 119, 125-126, 149, 156, 160-163
cell structure, 86-87
crude-oil-eating, 136
DNA, 88
evolution, 95
gene promoters, 164
genetically engineered, 3
herbicide degrading, 275-276
horizontal gene transfer to humans, 159-163
human consumption of, 184
hybrids, 113
natural genetic engineer, 123-127
nitrogen-fixing, 50, 95, 123, 148, 257, 259, 272
patents on, 135-136
reproduction, 5, 112, 124, 143
root-protecting, 272
thermophilic, 122
transcription and translation in, 89-90
Bacteriophages, 79-80, 112-115, 117, 118-120
M13, 157-158
Bailey, Liberty Hyde, 20-21
Balfour, Eve, 246
Barley, 5, 17, 18, 19, 24, 42, 98, 198, 278
Barry, Gerard, 296
Beachell, Hank, 65
Beachy, Roger, 129-132, 133, 138, 153, 164, 290, 291, 293
Beadle, George, 34-35, 37, 38, 79, 97, 98, 99
Beard, John, 184, 297, 298, 299
Beltsville Agricultural Research Center, 142
Berthaud, Julian, 242
Bertsch, Walter, 12
Bessey, C. F., 68
Beta carotene
in rice, 1-8, 9, 20, 66, 73, 126-127, 149, 152-153, 163, 184
Beyer, Peter, 4, 6, 7, 152-153
BIO, 240-241
Biodiversity, 315
gene flow and, 240-244
herbicide-tolerant crops and, 283-284
Biodynamic agriculture movement, 246, 257
Biofortification of crops, 296-300, 303
Biolistics Inc., 138
“Biotechnology” term
and public opinion, 287
Blackberry, Iceberg, 1, 8, 20, 54
Bob’s Red Mill Natural Foods, 16
Bolivia
organic farming, 260-261
water wars, 263
Borlaug, Norman, 47, 63-64, 65, 66, 183, 264, 265, 300, 311
Bouis, Howarth, 297
Boussingault, Jean-Baptiste, 50
Boyer, Herbert, 109-110, 119, 125, 127, 142, 161
See also Rape and rapeseed
Brassica oleracea, 69-71, 107, 239
Brassica rapus, 239
Braun, Armin, 124
Breeding. See Selective breeding
Bridges, C. B., 78
Broccoli, 69, 70, 72, 73, 217-218
Brookhaven National Laboratory, 9
Bt crops and pesticides, 292
gene flow, 233
grower/stewardship agreements, 218-220
and Monarch butterfly, 203-209, 210, 211
pest resistance to, 209-212, 213, 214, 215-221, 295-296
pesticide usage, 211-212
refugia requirements, 215-221
testing for toxicity, 193
and yields, 212
Burbank, Luther, 1, 4, 8, 13, 20, 51-54, 55, 56-58, 62, 105, 134-135, 165, 175
C
C4 photosynthetic pathway, 307
C. elegans, 123
California Institute of Technology, 79
CaMV 35S promoter, 131, 133, 137, 147, 163-168, 174, 224, 225, 227, 229, 230, 233
Cancer, 164-167, 210, 251, 253-254
Canola, 71, 73, 107-108, 151, 152, 169-170, 234, 236-239, 276, 277, 283
Carbon-fixing enzymes, 306-307
Carlsberg Research Laboratory, 19
Carnegie Institution of Washington, 57, 101
Carson, Rachel, 247
Cauliflower, 1, 69, 70, 71, 167-168
Cauliflower mosaic virus, 131, 147, 166-168
Celery picker’s rash, 210
Center for Science in the Public Interest, 220, 313
Centers for Disease Control (U.S.), 196, 256
Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), 224, 230, 233, 241, 242, 243, 297
Cetus Corporation, 120, 121, 122
Chakrabarty, Ananda, 135-136, 137
Chang, Annie, 110
Chapela, Ignacio, 223-230, 233, 240, 241-242, 243
Chapman, John. See Johnny Appleseed
Charles, Prince of Edinburgh and of Wales, 6, 60, 247
Cheese, 111-112
Chemical mutation breeding, 16-18, 30, 62, 96, 148, 249
Chickpea, 168
Chicory, 276
Chilton, Mary-Dell, 124, 125, 129, 130
Chimeras, 109-127.
See also Grafting
Chocolate, 24
Chromosome doubling technique, 16.
See also Colchicine
Chromosomes, 76, 77, 88, 96, 97, 99, 104.
See also DNA;
Genes
Chymosin, 111-112
City University of New York, 3
Classification of cultivated species, 67-69
Cloning. See also Gene transfer;
Grafting;
Tissue culture cloning
high school experiments, 118-119
public fears, 142
viral coat protein sequence, 130, 153
Coat protein-mediated resistance, 130-131, 138-140, 153, 163-168, 284-285, 290
Cocking, E. C., 13
Coconut milk, 11-12
Codex Alimentarius Commission, 252-253
Codon optimization method, 291
Codons and anticodons, 80-81, 89-90, 94, 123
Coffee, 24, 58, 168, 254, 311-312
Cohen, Mark Nathan, 43
Cohen, Stanley, 109-111, 119, 125, 127, 142, 161
Colchicine, 16-17, 30, 62, 96, 249
Cold Spring Harbor Symposium, 101
Cole, Herb, 171-172
Collins, G. N., 32-34
Color Additives Amendment, 148
Colorado Agricultural Experiment Station, 278
Colorado State University, 172, 173, 211
Columbia University, 76
Columbus, Christopher, 32
Columbus, Diego, 41
Connecticut Agricultural Experiment Station, 61, 265
Conservation Technology Information Center, 271, 273
Conservation tillage, 271-274, 277
Consumers Union, 161, 164, 173, 251
Conway, Gordon, 264, 265-266, 311
Cook, Jim, 183, 191, 214, 267, 269-270, 273, 275, 277, 278
Cooperative Research Center for Australian Weed Management, 238
Coordinated Framework for the Regulation of Biotechnology, 146
See also Maize;
Teosinte
allergens, 190, 191-196, 197, 198
Attribute, 208
bans on planting transgenics, 6, 230
Black Mexican Sweet, 100-101
Bt, 148, 151, 156-157, 191-196, 197-198, 202-209, 211-212, 218-221, 224, 227, 229, 241
C4 gene, 307
Copper Cross, 61
event 176 hybrid, 208
evolution and domestication, 32-41
fumonisins (toxins), 197-198
gene flow, 223-235
Golden, 296-297
herbicide-tolerant, 15, 18, 151, 275, 276, 277, 283
high-protein, 296-297
hybridization, 57-62, 73, 76, 98-99, 234-235, 313
hydroponically grown, 10
Intellicoat Early Plant, 151-152
KnockOut, 208
molecular methods used on, 142, 167
NatureGuard, 208
Roundup Ready, 227
transformation of, 138
transposons in, 97-105
wet milling, 196
Cornell University, 11, 20, 34, 97, 98, 127, 138, 140, 204, 207, 209, 217, 237, 298, 299
Correns, Carl, 73
Cotton, 6, 151, 152, 209, 218, 219, 276, 292
Council for Biotechnology Information, 7, 149
Creighton, Harriet, 98
Cross-hybridization, 234, 238.
See also Gene flow
Cross-protection, 130, 131-132
Cry proteins, 192-194, 195-196, 203, 205, 206, 207-208, 214, 225
Cryptic variation, 39-40
Cucumber mosaic virus, 281, 287
Cyanobacteria, 87
Cytokinins, 12
D
2,4-D, 275
Daisies, 25
Damsteegt, Vernon, 282
Darwin, Charles, 1, 11, 48, 50-51, 55, 56, 72, 75, 76, 102
Datta, Karabi and Swapan, 5, 20, 66
Davenport, Charles, 57
De Vries, Hugo, 16, 56, 57, 73, 74-75, 77
Developing countries
big-business concerns of, 292
food security in, 5, 8, 260-261, 267, 288-289
rejection of GM foods, 309-310
Diamond, Jared, 26-27, 32, 42, 43
Diamondback moths, 217-218, 219
Diploids, 28
DNA. See also Chromosomes;
Genes;
Genomes
chimeric molecules, 109-110
codons and anticodons, 80-81, 89-90, 94, 123
copying process, 36, 80, 88, 120-123
cross-species comparison, 85, 86-87, 159-160
discovery, 79
fingerprinting, 116
herring sperm, 11-12
homologous recombination, 113, 114
human daily consumption from food, 155
introgression, 224-225, 226, 241, 244
“junk,” 82-83
patents on cloned sequences, 137
plant, 167
recombination hotspots, 165
satellite, 82-83
DNA Plant Technology Corporation, 91
Dobzhansky, Theodosius, 69
Doerfler, Walter, 157-158
Domestication of wild species, 23, 25-26.
See also Evolution
crucial alleles, 43-44
Donald Danforth Plant Science Center, 132, 198, 291, 292, 298
Double-cross method, 61
Double fertilization, 232
Dow AgroSciences, 205, 212, 219, 297
Duchy Originals, 247
Dussoix, Daisy, 115
E
Ecogene, 269
Ehrlich, Paul, 301
Emerson, Rollins, 33-34, 37, 99-100
Environmental Defense Fund, 212
Epigenetic changes, 104-105, 181
Ereky, Karl, 60
Esherichia coli, 85, 110, 111, 113, 142, 180, 255
Euchleana mexicana, 32, 40, 67.
See also Teosinte
Euchleana parviglumis, 40
European corn borer, 193, 197, 211-212, 213, 215, 216, 218, 295
European Union, ban on GM food, 6, 224, 230, 277, 310-311
Evans, Lloyd, 45
Evolution
bottleneck, 30
evidence of species relationships, 85-91, 95
farming practices and, 28, 30, 31-32, 37-38, 42, 43
molecular clock, 39
F
F1 generation, 73-74
Fagan, John, 195
Farm Scale Evaluations (U.K.), 283
Federal Food, Drug, and Cosmetic Act, 148
Federal Insecticide, Fungicide, and Rodenticide Act, 147
Federal Interagency Committee on Recombinant DNA Research, 144
Federal Plant Pest Act of 1957, 146
Federici, Brian, 192, 193, 197
Ferreira, Steve, 139
Fertilizers, 49-50, 63, 248-249, 257-259, 276
Fire-colored beetle, 93-94
Firestick farming, 25
Fish genes in tomato, 91-94, 187
Fitch, Maureen, 139
Folate, 298
Food Additives Amendment, 148
Food and Agriculture Organization of the United Nations, 161, 187, 189, 190, 253, 303
Food and Drug Administration (U.S.), 6, 148-149, 169, 170, 173, 188, 195, 255
Food First, 225
Food poisoning, 180, 254, 255, 256
Food safety. See also Safety of GM crops
bacterial threats, 254-256
irradiation and, 248, 250, 256
Food security. See also Sustainable agriculture
consumption and waste issues, 267-268, 302-303
in developing countries, 5, 8, 260-261, 267, 288-289
economic issues, 310-311
per person availability, 303
population growth and, 302-303
Forster, B. P., 17
Fraley, Robert, 130-131, 132, 290
Fredrickson, Donald, 143-145
Freed, David, 180
French Revolution, 24
Frick, Oscar, 198
Friends of the Earth, 195, 196, 204, 207
Fumonisins, 197-198
G
Galanthus nivalis agglutin, 179
Galls, 123-124
Gaut, Brandon, 39
Gauthieret, Roger, 11
Gel electrophoresis, 116, 122, 225
Gene flow
and biodiversity, 240-244
from GM crops to landraces, 223-230, 240-244
pollen dispersion and, 231-233
Gene pools of crops, preservation, 241-242
Gene promoters, 4, 82, 130-131
CaMV 35S, 131, 133, 137, 147, 163-168, 174, 224, 225, 227, 229, 230, 233
Gene stacking, 238-239
Gene transfer, 5.
See also Cloning;
Recombinant DNA technology
horizontal, from bacteria to humans, 155-163
in nature, 124-127
procedures in plants, 109-110, 129-132, 138, 139
rates in brassicas, 236-237
transduction, 112-115
vectors, 83, 112-115, 109-110, 124-127, 129-131, 139
Genentech, 111
General Electric, 135-136
Genes
antisense, 92
cross-species comparison, 85-86, 96, 102, 103, 159
digestion process, 155-159
early concepts, 75-76
linkage groups, 78-79
mapping, 78, 79, 97, 98, 101, 102
naming, 37
one-gene, one-enzyme hypothesis, 34, 79, 98
retrotransposons, 102
sequencing, 123
silent, 103
transcripts, 82
transposons, 36, 83, 97-105, 125, 164, 174
Genetic engineering. See also Molecular breeding methods;
Recombinant DNA technology
in nature, 123-127
Genetic ID, 195
Genetically modified (GM) food plants. See also Molecular breeding methods;
Recombinant DNA technology;
Safety of GM crops;
individual crops
acreage planted in, 151, 152, 211-212, 221, 230-231
bans on, 6, 224, 230, 277, 310-311
crucial alleles, 43-44
donated by multinational corporations, 152-153, 291, 293, 296, 297
economic issues, 152-153, 215, 286-287, 288, 310-311
field trials, 140, 171, 173, 183, 283-284, 291, 293
and food security in developing
gene flow to landraces, 223-230
genetic stability of crops, 174-175
inputs for growing, 6-7
licensing restrictions on cultivation, 151-152, 194
multinational corporations vs. small family farmers, 151, 152-153, 292
myths and misconceptions, 7, 8, 9-10, 15, 71-72, 91-92, 155, 163-168, 170, 187, 285-287, 292, 293, 313-314
natural mutations, 1, 9, 26-27, 30, 31, 36, 37-38
nutrient content and bioavailability, 4, 183-184, 296-300
organic farming and, 249
public opinion on, 311-314
safety-enhancing attributes of, 197-199, 210, 285-286
and substantial equivalence, 148-149, 170-171, 173
yield-improving strategies, 303-309
cross-species comparison, 85-86
exons and introns, 82
extra copies, 96
human, 28, 39, 82, 85, 102, 159-160
sequencing, 81-82, 123, 124-125, 304
Genotype, 75
Genus, 68
Gildow, Fred, 281
Giles, W. F., 71
Glufosinate, 151, 238, 276, 283
Glycoalkaloids, 44-45, 168, 171-172, 181, 209
Glyphosate, 15, 151, 238, 240, 271, 274-275
GM Science Review Panel (U.K.), 162
Goat grass. See Aegilops spp.
Goklany, Indur, 264
Goldburg, Rebecca, 212
Gonsalves, Dennis, 138, 139, 150, 152, 286
Goodman, Howard, 110
Goodman, Major, 242
Grafting, 52-53, 108-109, 112, 127
Green Revolution, 3, 5, 41, 64, 264, 265-266, 300, 301, 304
Greenhouse gases, 271-272
Greenpeace, 187, 188-189, 204, 207, 299
Gregario, Glenn, 298
Gregory, W. C., 19
H
Haas, Jere, 298
Haber, Fritz, 259
Hansen, Michael, 164, 165, 173, 174
Hanson, Victor Davis, 201-202, 210-211, 219, 220, 263
Haploids, 231
Hardin, Garrett, 302
Harlan, Jack R., 23, 27, 29, 30-31, 34, 168, 214, 215
Harvard University, 10, 33, 34, 35, 311
Harvest index, 65
Hawaiian Agricultural Research Center, 139
Health Canada Food Directorate, 170
Health effects. See also Allergies and allergens;
Cancer;
Toxins in foods
agricultural development, 43
of conventionally bred plants, 19
horizontal gene transfer from bacteria to humans and, 159-163, 184
hormesis phenomenon, 253-254
of pesticide residues, 253-254, 276
Heiser, Charles, 60
Hemoglobin, in plants, 95
Herbicide-resistant weeds, 237
Herbicide-tolerant crops, 15, 17-18, 151, 163, 233, 237-238, 270-271, 273-278, 283-284
Heredity
dominant and recessive traits, 74, 215-217
fruit fly experiments, 77-78
genes and, 74-79
manikin theory, 76
Mendel’s laws, 55-56, 57, 58, 59, 60-61, 73-74, 75, 76-77, 215-217
Herman, Eliot, 198-199
Herrera-Estrella, Luis, 309
Herring sperm DNA, 11-12
Hershey, Al, 79-80
Heterosis, 61
Heterozygous, 59
High-fructose corn syrup, 111
2S High-methionine albumins, 189, 190-191, 197
Ho, Mae-Wan, 7, 163-167, 174, 225, 285
Hokkaido University, 13
Homologous recombination, 78, 113, 114
Homozygous, 59
Hormesis, 253
Horsch, Rob, 125, 131, 290, 291, 292, 296, 297, 299
Howard, Albert, 245-246, 248-249, 250, 251, 257, 258, 266
Hybridization. See also Chimeras
of bacteria, 113
chemical mutation methods, 16, 30
double-cross method, 61
genetic stability of hybrids, 173-174
grafting, 52-53, 108-109, 112, 127
intergeneric, 9, 16, 28, 53-54;
see also Species
by protoplast fusion, 13-14
wide crosses, 18-19, 28, 105, 173
I
Ice-minus bacterium, 145-146
Iltis, Hugh, 38-39
Imidazolinone, 15, 238, 276-277
Immunoglobulin E, 185-186, 189, 191, 196
Institute of Plant Industry (India), 245
Institute of Plant Sciences (Switzerland), 5
Institute of Science in Society, 7, 163
Integrated farming, 260
International Botanical Congress, 7, 315
International Conference of Plant Breeding and Hybridization, Second, 56
International Food Policy Research Institute, 208, 260-261, 297
International Potato Center, 290
International Rice Research Institute (IRRI), 3, 20, 64-65, 241, 296, 298
International Service for the Acquisition of Agri-biotech Applications, 150-151
Introgression, 224-225, 226, 241, 244
Irradiation of foods, 248, 250, 256, 314
Iowa State University, 3, 16, 61, 187, 212
Isoflavone, 172-173
Italy, San Marzano tomato, 279, 286, 287
Iwanaga, Masa, 224, 230-231, 233, 243
J
Jack-in-the-Box food poisoning, 255, 256
Jacob, François, 85, 86, 94, 95
James, Philip, 177-178
Jennings, Peter, 3
Johannsen, Wilhelm, 75
Johnny Appleseed, 52-53, 60, 72
Johns, Timothy, 45
Jones, Donald F., 60-61
Juma, Calestous, 311-312
Jumping genes. See Transposons
K
Karmate (tomato-potato hybrid), 13
Karp, David, 188
Kellogg, John Harvey, 188
Kenya
Agricultural Research Institute, 289-290, 291, 292
maize production, 260-261
virus-resistant sweet potato, 288-294
Khush, Gurdev Singh, 65-66
Kinetin, 12
Kinoshita, T., 13-14
Kishore, Ganesh, 275
Kiwi fruit, 187-188
Kraft Foods, 195
Ku, Maurice, 307
Kucinich, Dennis J., 10, 20, 60, 72
Kumeyaay, 25
L
La Guardia, Fiorello, 134-135
Lamarck, Jean-Baptiste, 72
Landrace conservation, 98-99, 223-230, 233, 241-243
Lauter, Nick, 40
Lawes, John Bennett, 49-50, 257
Lederberg, Joshua, 112, 113, 114, 143, 154
Lemaux, Peggy, 198
Leopold Center for Sustainable Agriculture, 206
Lettuce, 18, 20, 184, 255, 276
Levy, Avraham, 174
Liebig, Justus von, 49, 50, 257
Lima beans, 168
Lindemann, Julianne, 146
Lindow, Steven, 146
Linnaeus, Carl, 68
Listeria, 255
Lobitz, Robert, 1
Losey, John, 204-205, 206, 207, 209
Louis XVI, King of France, 23
M
MacLeod, Colin, 79
MacNeish, Richard, 35
Mad Cow disease, 246
Maize. See also Corn
archaeological discoveries, 40-41
classification of species, 67
evolution and domestication, 32-41
genetics experiments, 97-101
landrace conservation, 98-99, 223-230, 233, 241-243
teosinte crosses, 32, 34-35, 40, 223
Tripsacum crosses, 34
Makoto, Matsuoka, 65
Mangelsdorf, Paul, 34-35
Manioc, 168-169
Manshardt, Richard, 139
Margulis, Lynn, 159-160
Martineau, Belinda, 92
Martinez-Soriano, Juan Pablo, 233
Mayr, Ernst, 68
McCarty, Maclyn, 79
McClintock, Barbara, 83, 97-105, 164
McFadden, Sam, 30
Melchers, Georg, 13
Mendel, Gregor, 55-56, 57, 58, 59, 60-61, 73-74, 75, 76-77, 81, 102, 215-216
Messenger RNA, 89-90
Mexico
dwarf wheat, 63-64
maize, 40-41, 223-230, 233, 236
Mills, Wilford, 171-172
Molecular breeding methods. See also Recombinant DNA technology
conventional breeding methods
compared, 15, 19, 20, 60, 71-72, 78, 171-172, 182-183
culling of rejects, 165-166
disease resistance in crops, 128-132
marker genes, 4, 149, 156, 160-163
mutation breeding methods compared, 9-10, 19, 20, 78, 108, 141, 238
regulatory oversight, 140-150
“release experiments,” 145-146, 147
safety precautions, 20, 66, 142, 146
toxicity testing, 193
Molecular clock, 39
Monarch butterfly, 203-209
Monsanto, 15, 18, 92, 125, 130, 131, 133, 138, 140, 151, 152, 172, 173, 205, 208, 212, 219, 227, 273, 274, 275, 276, 290, 291-292, 293-294, 295, 296, 297, 313
Morgan, Thomas Hunt, 76-77, 78
Mori, K., 13-14
Morrison, L. A., 67-68
Morrow, John, 110
Motoyui, Ashikara, 65
Mouse-ear cress. See Arabidopsis thaliana
Muller, Hermann Joseph, 16
Mullis, Kary, 120-123
Mutant Variety Database, 19-20
Mutations and mutation breeding, 56, 62.
See also Somaclonal variation chemical methods, 16-18, 30, 62, 96, 148, 249, 277, 283-284
crop successes, 9, 16-18, 20, 62, 238
cryptic variation (hidden mutations), 39-40
database of varieties, 19-21
deleterious, 242
epigenetic changes, 104-105, 181
farming practices and, 26-27, 28, 30, 31-32, 37-38, 42, 43, 51, 209, 210
fruit fly experiments, 77-78
and human health, 19
molecular breeding methods compared, 9-10, 19, 20, 78, 108, 141, 238, 277-278, 283-284
in nature, 1, 9, 26-27, 30, 31, 36, 37-38, 53, 81, 96
radiation methods, 9-10, 14-16, 105, 141, 256
and regulatory oversight, 18-19, 141, 148, 283-284
transposons and, 36, 83, 102-103, 105
unstable, 99-101
Mwanawasa, Levy, 309-310
N
National Academy of Sciences (NAS), 98, 99, 101, 142, 149
National Agricultural Biotechnology Council, 112
National Center for Food and Agricultural Policy, 212
National Coalition Against the Misuse of Pesticides, 249
National Corn Exposition, 60
National Corn Growers’ Association, 313
National Institute of Agrobiological Resources, 307
National Institutes of Health (NIH), 143-144, 186
National Organic Standards Board, 248, 249, 250
National Plant Germplasm System (U.S.), 241
National Polytechnic Institute (Mexico), 233, 309
Natural and health foods. See also Organic farming and foods
breeding methods, 9, 16, 19, 108
Natural selection, theory of, 51, 72-73, 86.
See also Evolution
Nature (journal), 7, 82, 204, 206, 224, 225, 226, 227, 228, 230
Nature Biotechnology (journal), 240-241
New York Times, 7, 8, 204, 224, 228, 230, 252, 263
Nitrogen
fertilizers, 49, 50, 258-259, 304-305
Nitrogen-fixing
bacteria, 50, 95, 123, 148, 257, 259, 304-305
genes, 305-306
NOS terminator sequence, 225, 226
O
Ohio State University, 297
Olives and olive oil, 168, 169
Open University (U.K.), 163
Operons, 125
Oregon Agricultural Experiment Station, 278
Organic farming and foods
biotechnology and, 249, 250, 269-274
conventionally grown foods compared, 251-256
costs issues, 250-252, 259-260
in developing countries, 260-261
market for, 247
Organic Rule and, 248-251, 256, 259, 266, 271
and pesticides, 192, 202, 209, 214, 217, 247, 251-252, 254, 255
plant breeding techniques, 17
safety of, 254-256
soil fertility and, 246, 257-259
sustainability, 246, 259-261, 264, 266, 268-269, 270
testing, 249
yields, 260
Organic Food Production Act, 248
Organisation for Economic Cooperation and Development, 179, 183
P
Paarlberg, Robert, 155, 168, 169, 289, 292
Padgette, Steve, 275
Papaya, 132, 138-140, 149, 150, 151, 153, 309
Papaya ringspot virus, 138-140, 285
Pariza, Michael W., 209-210
Parmentier, Antoine Augustin, 23-24, 44, 45
Parrot, Wayne, 223
Patents, 133
agricultural development, 49, 133-134
on bacteria, 135-136
on cloned DNA sequences, 137
gene promoters, 131
hybridization method for wheat, 141
and licensing of technology, 138, 140, 151-152
standards for living organisms, 133-137
trade secrets compared, 133
virus resistance techniques, 132, 290
PCR. See Polymerase chain reaction
Peanut, 19, 185, 186, 187, 188, 189, 190, 198
Pennsylvania State University, 171, 172, 184, 281, 298
Pesticide Amendment (1954), 148
Pesticides. See also Bt crops and pesticides;
Herbicide-tolerant crops;
specific products
mechanisms of action, 274, 275, 276-277
natural, 254
and nontarget species, 201-209
organic farming and, 192, 202, 209, 214, 217, 247, 251-252, 254, 255
residues in food, 251-253
risk assessment, 252-253
Pew Initiative on Food and Biotechnology, 207, 313
Phenotype, 75
Photosynthesis, 11, 48-49, 198
carbon-fixing enzymes, 306-307
Phytohemagglutini, 180
Pinstrup-Andersen, Per, 129, 208-209, 260-261
Pioneer Hi-Bred Corn Company, 61, 62, 212, 313
Pioneer Hi-Bred International, 190-191, 212, 234, 297
Plant cells, 87-88
Plant hormones, 10-12.
See also Auxins;
Cytokinins
Plant-incorporated protectant, 148
Plant Protection Act of 1930 (U.S.), 135, 137
Plant Quarantine Act of 1912, 146
Plant sex, 5.
See also Gene flow
Plant Variety Protection Act (U.S.), 135, 137
Plasmids, 109-110, 112-113, 117-118, 119, 123, 124-127, 136, 142, 214
Plum, 53, 132, 186, 280, 282, 284-285
Plum pox virus, 280-281, 284-285
Pollination. See Gene flow;
Plant sex
PolyA tail, 89
Polygalacturonase, 92
Polymerase chain reaction (PCR), 120-123, 157, 195, 225, 226, 228-229
Polysaccharides, 93
Population growth
agricultural development and, 42-43, 62-63
and arable land, 266-267
ecological footprint, 267
and food security, 302-303
Malthusian principle, 47-48, 301
and species losses, 301
Post-transcriptional gene silencing, 131-132, 285
Potatoes
Delta Gold, 171
growing conditions, 44
herbicide-tolerant, 276
hybridization, 44
irradiation, 256
myths and superstitions, 23
origin, 45
patentability, 134
pest-resistant, 177
“poisoned rat debate,” 177-183
toxins, 44-45, 168, 171-172, 177-183, 209
Potrykus, Ingo, 1-2, 4-8, 20, 62, 66, 126-127, 152-153, 163, 184, 279, 299-301, 304
Proceedings of the National Academy of Sciences, 206-207, 208
Prokaryotes, 96
Proteins, 79.
See also specific proteins
cross-species similarities, 90-91, 94, 95
DNA methylation and binding to genes, 105
domains, 90
fluorescent, 162
folding, 90
hemoglobin, 95
mitochondrial, 87
most abundant, 306
therapeutic, 120
transcription and translation process, 88-90, 91, 94
Protoplasts, 13-14.
See also Tissue culture cloning
Pseudomonas fluorescens, 272
Pseudomonas putida, 136
Pseudomonas syringae, 145-146
Puccinia, 214-215.
See also Rusts
Purdue University, 271
Purnell, Fred S., 134-135
Pusztai, Arpad, 177-183
Q
Qaim, Matin, 288
Quist, David, 223-230, 233, 240, 241-242
R
Radiation mutation breeding, 9-10, 14-16, 105, 141, 256
Rape and rapeseed, 107-108, 169, 236
Ravelonandro, Michel, 284
Raven, Peter, 7, 13, 243, 244, 261, 267, 315
Ray, John, 69
Recombinant DNA technology. See also Cloning;
Gene transfer;
Molecular breeding methods
chimeras, 109-127
gene amplification, 117-123
gene sorting, 116
gene splicing, 109-111, 117-118, 123
high school experiments, 116-120
medical contributions, 3, 111, 120, 143
NAS risk assessment, 149-150
NIH risk assessment and guidelines, 143-144
public fears, 142-143
Recombination hotspots, 165
Reeves, Robert, 34
Regulation of GM crops
conventionally bred crops compared, 18-19, 140-150, 278, 298
costs of compliance, 152-153, 215, 286-287
disposal of rejected plants, 141
EPA authority, 147-148, 207, 220
exemptions, 286
of field tests, 141, 146, 286-287
generally recognized as safe standard (GRAS), 169
of “new” organisms, 147-148
pest resistance management plan, 148, 215-221
process-oriented, 140-150, 153-154
product approval time, 150
and public fears, 142-143, 154, 314
substantial equivalence standard, 148-149, 170-171, 173
USDA authority, 146-147
Restriction enzymes, 109-110, 115, 116-117, 119, 127, 228-229
Rhoades, Marcus, 100-101
See also Oryza sativa
BR29, 20
Dee-geo-woo-gen, 65
dwarf varieties, 64-65
Golden (beta carotene), 1-8, 9, 20, 66, 73, 126-127, 149, 152-153, 163, 184, 296, 299-300
Indica-Japonica hybrids, 69
IR36, 65-66
IR68144 (iron-rich), 298-299, 300
lodging, 65
nutrient bioavailability, 184, 298
radiation mutation breeding, 17, 20
pest resistance, 13-14, 65, 179
Peta, 65
salt-tolerant, 20
species and varieties, 2, 6-7, 69
tissue culture cloning, 12-15
virus resistance, 132
Rieger, Mary, 238
Rockefeller Foundation, 2, 3, 4, 5, 63, 64, 292, 296, 311
Rockefeller Institute for Medical Research, 79, 124
Rogers, Stephen, 130, 132, 290
Rothamsted Research Institute, 50, 55, 271
Roundup (glyphosate), 15, 18, 138, 148, 151, 157, 163, 172, 240, 270, 271, 273-276, 277, 278, 277
Roush, Richard T., 220
Rowett Research Institute (Scotland), 177-178, 180
Rubisco (RuBP carboxylase), 306-309
Rural Advancement Foundation International, 8
Russell, Robert, 299
Rutgers University, 39
Ryan, Angela, 163
See also Triticale
S
Sachs, Eric, 295
Sachs, Julius von, 10, 48-49, 50, 85, 107, 257
Safety of GM crops
allergens, 4, 177, 180, 183-196
antibiotic-resistance gene markers and, 160-163
assessment of, 169-175, 177-183, 184, 191, 195, 269, 298-299
CaMV 35S gene promoter and, 163-168, 225, 228
cancer claims, 164-167, 168, 172
conventionally bred plants compared, 18, 187-188, 190, 254-255, 298-299
(GRAS) standards, generally recognized as safe, 169
and horizontal gene transfer, 155-16
methodological issues in studies, 157-159, 178-183, 204-205, 225-229
natural toxins and, 168-169, 177-183
for nontarget species, 202-209
organically grown crops compared, 254-256
pathogen use in recombinant DNA technology, 143-144, 163-168, 184, 213-214
“poisoned rat debate,” 177-183
substantial equivalence standard and, 170-171
Sakamura, T., 28
Sala, Francesco, 286
Salmon, S. C., 64
Saussure, Theodore de, 48, 257
Schimke, Robert, 110
Schubert, Karel, 298
Science (journal), 7, 82, 142, 177, 206, 225, 228
Scorza, Ralph, 284
Scottish Crop Research Institute, 17, 180
Sears, Ernest, 30
Sears, Mark, 208
Selective breeding. See also Hybridization;
Molecular breeding methods;
other specific methods
“natural” vs. “unnatural,” 8-21, 107, 129
for nutrition, 5
for virus resistance, 129-132
Sharka. See Plum pox virus
Shelton, Anthony, 205, 209, 212, 217, 220, 221
Shipak, Florence, 25
Shiyu, Leslie, 109
Shull, George Harrison, 57-59, 60, 61
Skoog, Folke, 11-12
Slightom, Jerry, 138
Slow Food movement, 286-287
Snowdrop, 177.
See also Galanthus nivalis agglutin
Society of Toxicologists, 160-161
Soil
acidification, 308-309
crumb structure, 272, 273, 274
ecology, 272-273
EPSP synthase in, 274-275
erosion, 270-271
fertility, 49, 245-246, 248, 250, 257-259
GM organisms, 183
nitrogen cycle, 257-258
nitrogen-fixing bacteria, 50, 257, 259, 272
organic farming and, 246, 257-259
root-protecting bacteria, 272
salinization, 307-308
streptomycete, 276
Solanum tuberosum, 45.
See also Potatoes
Somaclonal variation, 15, 141-142, 148, 181-182, 183, 277, 283-284.
See also Mutations and mutation breeding;
Tissue culture cloning
Soybeans, 275
allergens, 186, 190-191, 197, 198-199
isoflavone content, 172-173
nutrient content, 191
Roundup Ready, 138, 148, 151, 157, 172, 240, 270, 273-274, 275-276, 277, 292
Species
crossing barriers between, 71-78, 124, 127
See also Mutations and mutation breeding
Squash, 26, 38, 132, 138, 165, 168, 290
Stanford University, 18, 109, 110, 115, 145, 312
Stark, Paul, 134-135
Stebbins, Ledyard, 102
Stefansson, Baldur, 107
Steiner, Rudolf, 246
Steward, F. C., 11
Stone, Robert, 143
Streptomycete, 276
Sturtevant, Alfred, 77, 78, 101
Sugarcane, 276
Superphosphate of lime, 49-50
Sustainable agriculture
arable land and, 266-267
biotechnology and, 268-269
Bt crops and, 13, 202-209, 211, 215-221
consumption and waste issues, 267-268, 302-303
conventional farming methods and, 268, 295, 314-316
cost-effectiveness, 270
direct seeding, 273
herbicide-tolerant crops and, 270-271, 273-278
no-till methods, 270-274, 275, 277-278
organic farming methods and, 246, 259-261, 264, 266, 268-269, 270
organo-transgenic methods, 269-274
pest resistance management, 215-221, 278, 295-296
population growth and, 266-267
Sweet potato, 288-294
Sweet potato feathery mottle virus, 291
Syngenta Seeds, Inc., 7, 151, 205, 208, 219
T
Tabashnik, Bruce, 220-221
Takebe, I., 13
Taq polymerase, 122
Tatum, Edward, 79
Taylor, Steven, 183, 190, 191, 196
Tea, 168
Teosinte, 58
evolution of maize from, 32-41, 76, 223
geographical area, 33, 40-42, 236
modern maize compared, 33, 233-234
Tetraploids, 28
Texas A&M University, 1
Texas Agricultural Experiment Station, 278
Thomashow, Linda, 272
Tissue culture cloning, 139
anther method, 14-15
discovery, 12
growth-promoting factors, 10-12
and mutations, 13, 105, 181-182;
see also Somaclonal variation
protoplast fusion method, 12-14
and regulatory oversight, 141, 148
Tobacco, 5, 11, 13, 24, 126, 131
Tobacco mosaic virus, 130, 131
Tomato, 26, 91-94, 131, 132, 175, 276, 279, 287
Tomato-eggplant hybrid, 13
Tomato fern leaf virus, 279, 286-287
Tomoffel (tomato-potato hybrid), 13
Toxic Substance Control Act, 147
Toxins in foods, 44-45, 168-169, 171-172, 179-180, 182, 197-198, 209-210, 255
Transfer-DNA, 125, 131, 132, 174
Transfer RNA, 90
Transgene, 112
Transposons, 36, 83, 97-105, 125, 164, 174
Triticale, 9, 16, 19, 20, 62, 73, 96
Triticum turgidum, 28
Triticum urartu, 28
Tull, Jethro, 133-134
U
Union of Concerned Scientists, 204, 207
United Nations Environment Programme, 263
University College London, 31
University of Adelaide, 238
University of Arizona, 220-221
University of Arkansas, 198, 240
University of Bern Botanical Gardens, 9, 231, 235, 269
University of Bonn, 288
University of California
University of Cologne, 157
University of Geneva, 115
University of Ghent, 124, 125, 130
University of Hawaii, 139, 140
University of Illinois, 30
University of Leeds, 156
University of Liverpool, 3
University of Milan, 286
University of Nairobi, 290
University of Nebraska, 183, 190, 205, 268
University of Newcastle upon Tyne, 157
University of Washington, 124, 125, 213
University of Western Australia, 238
University of Western Ontario, 163
University of Wisconsin
Parkside, 25
Upjohn Company, 138
U.S. Agency for International Development, 264, 290, 292, 296
U.S. Department of Agriculture, 53, 64, 281
Bureau of Plant Industry, 32
Foreign Disease-Weed Laboratory, 282
National Organic Program, 248, 250
National Plant Germplasm System, 241
regulatory authority over GM crops, 146-147, 153-154, 195-196, 206, 220
state agricultural experiment stations, 55, 59, 61, 139, 142, 206
U.S. Environmental Protection Agency, 147-148, 153, 191, 193, 194, 207, 213, 214, 251, 286
U.S. House of Representatives Committee on Science, Subcommittee on Basic Research, 162
U.S. Navy, plant exploration expeditions, 54
U.S. Patent and Trademark Office, 55, 134, 136.
See also Patents
V
Vectors, 83, 109-110, 112-115, 124-127, 129-131, 139
Virus resistance
C-5 plum tree, 284-285
coat protein-mediated, 130-131, 138-140, 153, 163-168, 284-286, 290
codon optimization method, 291
papaya, 132, 138-140, 149, 150, 151, 153, 285-286
post-transcriptional gene silencing, 131-132
sweet potato, 288-294
Viruses, plant
coat protein, 130
human health effects of eating, 166-168
structure, 167
W
Wallace, Henry C., 61
Wambugu, Florence, 288, 289-292, 293-294, 295, 296
Wampanoag, 25
Washington State University, 64, 183, 259-260, 267, 272, 307
Washington University, St. Louis, 125, 129
Watermelon, 24
New Queen, 171
Weeds. See also Herbicide-tolerant crops
management in orchards, 282-283
no-till agriculture and, 273-274
resistance to herbicides, 238, 239-240
wide crosses with, 18-19, 28, 105, 173
Weizmann Institute, 174
Weller, Dave, 272
Went, Frits W., 11
Wettstein, Dieter von, 19
Wheat, 5.
See also Triticum spp.
Ceres, 215
classification of species, 67-68
drought resistance and cold
tolerance, 29
dwarf varieties, 63-64
evolution and domestication, 26-32, 42, 173-174
gene flow, 231
hard red winter, 17-18
harvesting techniques, 31-32
herbicide-tolerant, 17-18, 270-271, 276, 277, 278
Hope, 215
lodging, 63-64
Marquis, 215
nitrogen-fixing enzyme, 305-306
Norin, 64
rust-resistant, 64, 183, 214-215, 241
selective breeding methods, 9-10, 16, 17, 30, 141, 147, 148
Thatcher, 215
Wilkins, Maurice, 80
Wine grapes, 24, 201-203, 210-211
Wöhler, Friedrich, 49, 50, 257
Workshop on Safeguards for Planned Introduction of Transgenic Oilseed Crucifers, 237
World Bank Consultative Group on International Agricultural Research, 297
World Health Organization, 2, 161, 187, 189, 190, 253
World Resources Institute, 270
Worldwide Fund for Nature, 267
Wotecki, Catherine, 187-188
Y
Ye, Xudong, 127
Youatt, William, 51
Z
Zaire, arable land, 266
Zambia, rejection of GM food shipments, 309-310, 313
See also Corn;
Maize
Zeneca Agrochemicals, 7
Zimbabwe, rejection of GM food shipments, 309
Zohary, Daniel, 26
Zymotechnology, 111